The rapid propagation of sugarcane tissue culture seedlings in the field

In recent years, in order to accelerate the propagation of improved varieties, advanced biological technology has been used to organize tissue culture using sugarcane tissue or axillary buds. As the tissue culture seedlings grow weakly, the adaptability to the production environment is not strong in the early period, and the cultivation should be focused on standardized cultivation and fine management, with accelerated rapid propagation as the core and the purpose of rapid propagation of good varieties.
1 Standardized cultivation, fine management
1.1 Field selection: Choose the soil with good texture, flat terrain, fertile soil, convenient drainage and irrigation or irrigated land.
1.2 transplanting: fine soil preparation, do Tianping, ditch straight, fine soil. Field 100cm open planting ditch, irrigated to 95cm open planting ditch, playing pond planting, pond distance 33cm, acres of paddy fields with 2000 seedlings, irrigated to 2200.
1.3 Fertilizer and water management: 50 kg of Pu-calcium as the base fertilizer during planting, 15 days after transplanting, applying a thin fertilizer, 10 kg of urea per mu, promoting early growth of the cane seedlings, and maintaining soil moisture in the field (field) after planting. , 10 days after irrigation once permeable. Management after survival is the same as conventional cultivation.
2 field rapid propagation technology Sugarcane tissue culture seedlings planted the first generation of thin, multiple tillers, single bushes more effective stems, harvesting per acre effective stem is not less than the live, but the planting seedlings less, the production of more applications of thin tubes, In order to maximize the guarantee of tillering into stems, the rapid propagation of tissue culture technology will be widely used in production. At present, there are two commonly used methods:
2.1 repeated stem method:
When each bundle of about 3 to 5 stems extend to about 70-90 cm, and when the number of effective shoots per stem is 4-5, the main stem is cut down and the cane stems are transplanted for field transplanting. Before the next planting, drying for 1-2 days, when transplanting, adopt two buds under the trapezium to plant seedlings. The amount of buds under 1000-1500 buds will be buds. If the temperature is high in spring and autumn, the amount of rainwater will be higher, and the amount of seed can be reduced appropriately. Repeated harvesting of stems in such fields for transplanting in fields results in 30 times the reproduction rate of conventional field breeding.
2.2 stem taking method:
The method of taking stems and stems is to cut and plant the stems and shoots and plant the seedlings, and transplant and transplant the remaining tillers to achieve the purpose of increasing the breeding multiples of sugarcane varieties.
Cane stalk seedlings are planted in the same way as in Daejeon. However, when transplanting seedlings, they should bring more soil, less root damage, and remove old leaves. Two-thirds of each green leaf is cut, which helps to reduce water evaporation and increase the survival rate. Because the growth speed of tillers is different and the growth is different, the sorting treatment before transplanting is beneficial to the growth of seedlings to achieve uniform seedlings, seedlings, and seedlings, which will facilitate field management. Transplanting season should be selected before the rainy season or during the rainy season when the survival rate of transplanting is high, and the survival rate is low during the dry season; when transplanting, attention should also be paid to the timing of seedling cutting. If the seedlings are taken too late, the tillering seedlings have begun to decline. , resulting in malnutrition, low survival rate.

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