The suitable temperature for the growth of mycelium of P. ostreatus is 30-35°C. If you have air conditioning and heating conditions, or suitable natural conditions, cultivation can be carried out throughout the year. (A) The preparation of strains of the mother mushroom of Pleurotus eryngii can be obtained from its sclerotia and fruiting bodies for tissue separation. In the PDA medium, the mycelia of tiger puffball mushroom grows vigorously, is white, and has strong ability to climb the wall. At the end of the mycelium bundle, small sclerotia sometimes form. The production methods of the original species and cultivated species of tiger milk mushroom are similar to various woody rot fungi such as shiitake mushroom and oyster mushroom, and the wood bran medium (78 pieces of wood chips, 20 pieces of bran, 1 part of sugar and 1 part of gypsum powder) can be used. Material ratio 1:1.3-1.5) Preparation. (II) Wood cellar cultivation 1. The wood saws the trunk or coarse branches of broad-leaved trees such as alder, maple, eucalyptus, and poplar, and saws them into 30-45 cm sections. Sections more than 15 cm in diameter can be split, dried or dried. 2. The site is in the sunny slope or well-drained orchard, sunny digging 15-20 cm shallow pit, orchard soil is suitable for loam or sandy loam soil. 3. Inoculate the three pieces of wood and pour it into shallow pits in the shape of “goodsâ€. Place about 500 grams of woody smut species between the sections of wood and then cover with soil. 4.Manage soil in time to prevent wood from being exposed. When it is particularly dry, water or spray water to keep the soil moist. Regularly remove the weeds from the cultivation site. After 5 months, check whether sclerotia have formed around each pit. (3) Substitute cultivation 1. The commonly used culture ingredients for making cultivation bags are: (1) 78 kg of sawdust, 20 kg of wheat bran, 1 kg of sugar, 1 kg of calcium carbonate, and a ratio of material to water of 1:1.1-1.3. (2) 39 kg of wood chips, 49 kg of cotton seed shells, 10 kg of bran, 1 kg of sucrose, 1 kg of calcium carbonate, and a ratio of material to water of 1:1.1-1.3. After the culture material is prepared, a 17 cm x 35 cm polypropylene or low pressure polyethylene plastic bag is loaded. One end of the plastic bag is tied or welded, the other end is covered with a plastic collar, a tampon is plugged, and the kraft paper is wrapped. The sterilized and cooled inoculation is performed. 2. Cultivation Management Bags are inoculated and discharged on the bed frame of the cultivation room. The room temperature is controlled between 25-35°C. After 30-45 days of cultivation, the white mycelium was full of bags, and then the hyphae began to build up on the top of the culture medium or in the middle to form the nucleus of the mushroom. The sclerotia grow up. When the sclerotia is about to break the plastic bag, remove the plastic collar, unplug the tampon, and loosen the bag. When the culture material in the bag shrinks violently, the water comes out, becomes soft, and the sclerotia no longer grow up, it can be gradually harvested. 3. The sclerotia of the sclerotia mushroom after harvesting should be air-dried or hidden in wet sand. In the late spring and early summer, the sclerotium is first immersed in clean water, and the sclerotia sucks enough water to place it on a sand bed. The sclerotia can produce fruit bodies one after another. It takes about 7 days from the appearance of the primordia to the maturation of the fruiting body. If the temperature is low, the nucleus will have a longer fruiting body. 4. After processed and eaten, the nuclear of the mushroom is harvested, washed with water, and then cut into slices of 1-2 mm in thickness or dried or air dried, or dried in a dryer. Although the fruit body of Mushroom is edible, its texture is tough and its taste is not fresh, so its main use is its sclerotia. The dried sclerotia are ground into a fine powder with a crusher, and the powder of the tiger sphagnum nucleus nucleus can be made into cakes with rice flour, flour, sugar and the like. Ningbo DOKEE Medical Technology Co., Ltd. , https://www.dokeemedical.com