Precautions and cleaning methods for cuvettes

First, the cuvette notes cuvettes are generally rectangular, the bottom and sides are frosted glass, the other two sides of the optical glass made of light-transparent surface is melted, glass powder high-temperature sintering and glue bonding. Therefore, you should pay attention to the following points when using::
1. When taking the cuvette, only touch the frosted glass on both sides with your fingers to avoid contact with the optical surface. At the same time, pay attention to light handling, to prevent the influence of external force on the contrasting plate, and damage after stress.
2. Any solution containing corrosive glass shall not be placed in the cuvette for a long time.
3. The cuvette cannot be placed on a flame or an electric furnace for heating or baking in a dry box;
4. When the inside of the cuvette is found to be contaminated, wash it with absolute ethanol and wipe it clean in time.
5. Do not touch the light-transmissive surface of the cuvette with hard objects or dirt. When the solution is contained, the height is 2/3 of the cuvette. If there is residual liquid on the optical surface, it can be gently absorbed by the filter paper and then wiped with lens paper or silk.
Second, the cuvette group test is suspected in the comparison of the color plate when measuring, you can detect it yourself, the method is as follows:
1. The user can set the wavelength to the wavelength actually used, inject a set of cuvettes into distilled water, adjust the transmittance of one of them to 100%, measure the transmittance of each other, and the difference of the transmittance. Not more than 0.5%, it can be used together.
2. Before the colorimetry, put each cuvette into distilled water and compare it at the colorimetric wavelength. 4-8 of the cuvettes with an error of ±0.001 absorbance are selected for colorimetric determination, which avoids the cuvette. The difference causes measurement errors.
3, my company's cuvette, tested before leaving the factory, in principle, 0% error.
Third, the washing method of the cuvette with the rapid development of the spectrum analysis instrument, traces, semi-micro, fluorescence and other cuvettes continue to appear, the use of maintenance, cleaning cuvettes have higher requirements. Generally used in a single-use abroad, in order to save costs, open source and reduce the use of re-use. How to compare the color dish for cleaning, only according to various reagents, using the method of dissolving and neutralizing, in principle, the structure and light transmission performance of the cuvette cannot be damaged; the second is to use the method of neutralization and dissolution. Achieve the clean effect of the cuvette. The cleanliness of the cuvette in the spectrophotometer is one of the factors that affect the accuracy of the measurement. Therefore, we must pay attention to choosing the correct cleaning method. Here are a few ways to clean:
1. For example, if the solution is acid, if it is not clean, wash it with a weak alkaline solution. If the solution is alkali, if it is not clean, wash it with a weak acid solution. If the solution is organic, if it is not clean, use organic solvent. , such as alcohol and other solutions to wash.
2. The principle of selecting the cuvette washing solution is that the decontamination effect is good, and the cuvette is not damaged, and the measurement is not affected at the same time.
3. Analysis of commonly used chromic acid washing liquids is not suitable for washing cuvettes. This is because the water-carrying cuvettes sometimes locally heat up in the washing liquid, causing the cuvette bonding surface to crack and be damaged. At the same time, the cuvette washed by the washing liquid is likely to retain traces of chromium, which is absorbed in the ultraviolet region, thus affecting the determination of chromium and other related elements. It is generally recommended to wash with a mixed solution of nitric acid and hydrogen peroxide (5:1) and then rinse with water.
4. For the cuvette which is difficult to clean by the general method, the following two methods can also be adopted.
A. First, the cuvette is invaded by a solution of sodium carbonate (20 g/L) containing a small amount of anionic surfactant, washed with water, and then inhaled in a mixed solution of hydrogen peroxide and nitric acid (5:1). hour.
B. Wash in a fume hood with a mixture of hydrochloric acid, water and methanol (1:3:4), usually no more than 10 minutes.
C. Cuvettes should not be washed with lye, nor with hard cloth or brush.
Fourth, the above method is applicable to all kinds of cuvettes of the company's glass powder high temperature sintering.
5. Black wall micro quartz cuvettes, because of their inconsistent materials, especially should not be soaked in alcohol, after use, you can immediately use cotton balls or mirror paper to wash with alcohol, dry, and then stored in a clean Spare in the container or box.
Sixth, the molten one-color cuvette can refer to the cuvette cleaning method of high-temperature sintering of glass powder. It can be soaked in alcohol or acidic solution for a long time, or it can be cleaned by ultrasonic cleaning machine. The ultrasonic frequency should be adjusted to the minimum during operation to avoid The frequency is too high and the cuvette is broken.

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