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Rapid splicing method to isolate bacteria
Strains are the roots of edible fungi production, and its quality directly affects the yield and quality of mushrooms. Due to the limited technical conditions of most of the peasants, the mother species used are purchased from foreign countries and rarely separate and purify their own species. Even if they are separated, the operation is complex and the success rate is not high. To this end, a method for the isolation of strains is introduced - the pleating method. Select the first mature mushroom that has been unfolded in the cap. Cut approximately 1.5 cm of the pleats at 2/5 of the front of the pleats. Pick a piece of pleats with tweezers and affix them to the sterilized product. The slant medium is directly above and the test tube is then placed upright in a beaker or jar so that the spores can freely project onto the medium. When the surface of the culture medium is seen to have spore imprints, the bacteria pleats are taken out of the flame of the alcohol lamp in the inoculation box, and the test tube is put into an environment of about 25° C. After germination, the tube is purified and expanded in time. . The above operations should be performed aseptically.