Grafting of fruit trees is usually divided into spring and summer. In spring, branches are used (spiked joints), and the methods of grafting are mostly splicing, cutting, and plugging. In summer, budding is used more often. At present, it is a good time for fruit trees to be grafted in the spring. To improve the survival rate of grafting, the following five points need to be noted: 1. Grasp the grafting time. Grafting is early, the juice of the tree does not flow, the buds of the scions do not germinate, and it is not easy to survive after grafting. Grafting is late, branches have grown leaves, water evaporation, grafting is not easy to survive. In the middle of the Spring and South China Spring Festival, the pick-up is usually from late July to late August. 2. Select robust branches. The branches are robust, full of leaf buds, strong vitality, and easy to survive. 3. Avoid rain, windy weather. To listen to the weather forecast in time, select the higher temperature and sunny weather grafting. Generally, it is best if there is no heavy rain for 3 to 5 days after grafting. 4. Scion sealing wax. In order to reduce the water evaporation of the scion, it is necessary to seal the wax on the scion. The method is to melt the industrial paraffin. When the wax temperature reaches 95°C~102°C, cut the scion branches quickly and wipe it in the wax liquid quickly. Quickly click. 5. Grafting speed should be fast, tie bars should be tightened. The branches are best used with the scissors. Scraps that are not used on the same day are best placed in plastic bags in a cool, damp place. The cutters used for grafting should be sharp, the stripping surface must be smooth, and grafted in time. The long time is prone to dehydration and oxidation, which affects the survival rate of grafting. Disclaimer: Some articles in this website have been transferred from the Internet. If there is a third party's legal rights, please inform this website. phone Magnetic Bead Nucleic Acid Extraction Reagent
The magnetic bead method nucleic acid extraction kit is a high-tech product that combines biological science and nanomaterial science. It is a major breakthrough in my country's nucleic acid extraction and purification technology. .
Magnetic bead method for nucleic acid extraction has incomparable advantages over traditional DNA extraction methods, which are mainly reflected in: 1. It can realize automatic and large-scale operation. At present, there are 96-well nucleic acid automatic extraction instruments, and the extraction time of one sample can be realized. The processing of 96 samples complies with the high-throughput operation requirements of biology, enabling rapid and timely response to infectious disease outbreaks, which makes traditional methods unmatched; â‘¡ The operation is simple and time-consuming, and the entire extraction process has only four steps , most of them can be completed within 36-40 minutes; â‘¢ Safe and non-toxic, no toxic reagents such as benzene and chloroform in traditional methods are used, and the damage to experimental operators is minimized, which fully conforms to modern environmental protection concepts; â‘£ Magnetic beads and nucleic acids The specific binding of the extracted nucleic acid results in high purity and high concentration of the extracted nucleic acid.
According to the same principle as the silica membrane spin column, the superparamagnetic silica nano-magnetic beads are prepared after the surface of the superparamagnetic nanoparticles is modified and modified by nanotechnology. The magnetic beads can specifically recognize and efficiently bind to nucleic acid molecules on a microscopic interface. Using the superparamagnetic properties of silica-coated nanomagnetic microspheres, under the action of Chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate, etc.) and an external magnetic field, samples from blood, animal tissues, food, pathogenic microorganisms, etc. The isolated DNA and RNA can be used in clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial testing, food safety testing, molecular biology research and other fields.
Magnetic bead nucleic acid extraction can generally be divided into four steps: lysis-binding-washing-elution.
Genetic testing will surely become a new symbol of the development of the biological industry. The emergence of high-throughput, automated nucleic acid extraction methods will reduce the labor cost of genetic testing, make large-scale testing a reality, and make it possible for genetic testing to reach ordinary people.
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