UV detector overview: The ultraviolet detector is an ultraviolet device in liquid chromatography. The instrument is equipped with a chromatography column, a constant current pump, a partial collector, etc., which constitutes a complete liquid chromatography separation and analysis device, which is studied from modern biology. Quantitative analysis of samples with UV absorption by drug determination, agricultural research, chemical, food and medical units. The main components of the instrument are imported, and the photoelectric tube is IP28, with stable performance and high sensitivity. Main technical indicators of UV detector: UV detector works: UV detector instrument structure: The UV detector recorder is based on the contract signed by the user. At present, our company is equipped with XWD-100 long type recorder, LM17-A, LM14-104, XWT-1044 and 3057 desktop recorder. When he uses it, he will refer to the instructions. The company's instrument recorder has the following requirements: UV detector considerations
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1. Nucleic acid protein detector wavelength: 254nm, 280nm
2, UV detector wavelength: 220nm, 254nm, 280nm, 340nm
3, sample cell, volume 100 microliters, optical path 3 mm
4, range: 0-100% T, 0-2A, 0-1A, 0-0.5A, 0-0.2A, 0-0.1A, 0-0.05A
5, LED digital display
6, the instrument can work continuously for one week
7, power: 220VAC ± 10% 50HZ
8, temperature: -5 ° C ~ 35 ° C
The basis of the working principle of the instrument is the law of light absorption. The light emitted from the light source passes through the slit, the filter, and the sample cell to the photo-electric tube, so that the change of the light intensity caused by the difference of the sample concentration is converted into a change of the photocurrent, and the photocurrent is input to the logarithm through the amplifier. The converter converts the transmittance T into an A output, that is, A=1g — = εCL where ε is the molecular extinction system of the sample to be tested, C is the sample concentration, and the unit is gram/liter unit, and L is the optical path. Use centimeters as the unit. According to the above formula, it is known that A is measured, and the sample concentration C is known. If the recorder is directly input from the amplifier, the map of the change in the transmittance T of the sample is plotted. If the input from the logarithmic converter is input to the recorder, the spectrum of the change in the optical density A of the sample is plotted.
It consists of a set of light sources, four interference filters, a condenser lens, a sample cell, a photomultiplier tube, an amplifying plate and a logarithmic plate. The panel has a polyethylene plastic tube. Sample and outlet, A zero and adjust the “light†size knob (the amount of light is indicated by the arrow). There are also light source indicators, power indicator lights, and range conversion knobs.
The light source mainly provides 220, 254, 280, and 340 nm as light sources for detecting nucleic acids, proteins, enzymes, and polypeptides, respectively.
The color filter consists of lines of other wavelengths outside the 220, 254, 280, and 340 nm lines, and the other wavelengths other than the non-line wavelength are filtered by the corresponding color filters, which ensures the instrument's Monochrome.
The nucleic acid protein detector is the same as the ultraviolet detector except for the measurement of 254 nm and 280 nm.
The sample cell is made of quartz glass tube, and the inner diameter is generally about 3 mm, and the volume is not more than 100 microliters.
1. The range must be 10mv.
2. The paper feed speed is 3cm, 6cm or 12cm per hour.
How to use the UV detector:
1. First connect the host and recorder two parts of the circuit, then plug the host's power plug into the 220V power socket.
2. The wavelength setting before delivery is 280nm. If other wavelengths are needed, just turn the wavelength conversion knob on the right side of the instrument to dial the desired file.
3. Press the power switch indicator of the main unit to light. If the transmittance is 100%T, turn the knob (ie sensitivity) to the T position. Adjust the light knob and the LED digital display also displays the number.
4. If the optical density A test is performed, turn the range knob (ie sensitivity) to the T position. Adjust the light amount knob so that the LED digital panel display is around 98. The instrument is preheated for about 1.5 hours. When the baseline is stable, the instrument is calibrated.
5. Connect the plastic tube at the lower end of the column to the plastic tube of the host, and send the plastic tube to a part of the collector or test tube. At this time, the eluent passes through the sample pool.
6. Adjust the light amount knob, and set the range knob to 100% T file, so that the LED digital display is 100, T is set to A file, so the reading on the LED panel is about 0. If the transmittance is T test, it can be used in the column. The sample was loaded and the sample was separated and analyzed.
7. If the optical density A test is performed, turn the range knob (ie sensitivity) to the desired optical density A, and adjust the light amount knob so that the LED panel is 0. At this point, the recorder returns to near zero (note: near zero), you can customize it in the experiment.
8. If the peak of the plot is too small, turn the range knob (ie sensitivity) to the desired position.
9. During the test, the “light quantity†no longer changes. The test is completed and the sample cell and tubing are rinsed with distilled water.
1. The light intensity of mercury lamps is greatly affected by temperature and voltage. It is recommended that users work in a constant temperature room or work in an ice storage.
2, T-zero production time has been calibrated, when the photoelectric tube is replaced, it needs to be recalibrated.
3, mercury lamp life can reach thousands of hours, if you find that the recorder can not be adjusted to 10mV, and the baseline has been drifting in a small direction to consider changing lights. If the mercury lamp is not bright, press the power switch again, the mercury lamp will be on, and the photoelectric tube life will be more than several thousand hours. Be careful not to take out the sample cell holder when starting the machine, so as to avoid damage caused by strong light irradiation. When the mercury lamp light-emitting area and the photo-electricity tube photo-cathode window are stained, it can be wiped clean with a clean cotton core.