Reflective tape
Jerry tape has a wide range of reflective tapes. Our reflective tape has different kinds of materials, and they are suitable for diffierent applications. Blow are the ranges we have:
1) Advertisement grade Reflective Sheeting
Our advertisement grade reflective sheeting also have different serious: 3100, 3200, 5100, 5200, 7100 and 7200.
Which is based on PVC material, more bright, service life is 1~3 years.
3). High intensity grade reflective tape
Based on first class PET/ PC/ Acrylic material, adopted reflection principle of glass beads, has better reflective effect and longer service life. Also has DOT-C2 and Solas certificate.
4). Micro prismatic grade reflective tape
This grade of reflective tape is based on the Reflection principle of diamond prism, has perfect reflection and longer service life, can meet the application of freeway, heavy-duty vehicle, etc.
Reflective Tape,Reflective Strips,Reflective Duct Tape,Reflective Safety Tape Kunshan Jieyudeng Intelligent Technology Co., Ltd. , https://www.jerrytape.com
Human b-Amyloid 1-42 (b-Amyloid 1-42) ELISA kit instruction manual
Human b- Amyloid 1-42 ( b- Amyloid 1-42) ELISA kit
 ( used in serum, plasma, cell culture supernatants and other biological fluids )
principle
This experiment used double antibody sandwich ABC-ELISA. The anti-human b-Amyloid 1-42 mAb was coated on the plate, and the b-Amyloid 1-42 in the standards and samples was combined with the mAb, and biotinylated anti-human b-Amyloid 1-42 was added. The immune complex is formed on the plate, the horseradish peroxidase-labeled Streptavidin is combined with biotin, the substrate working solution is added blue, and finally the stop solution sulfuric acid is added, and the OD value is measured at 450 nm, b-Amyloid 1- The 42 concentration is directly proportional to the OD value, and the concentration of b-Amyloid 1-42 in the specimen can be determined by plotting a standard curve.
Kit composition ( 2-8 ° C preservation)
Coated Wells
96 holes
Enzyme Conjugate
12ml
10× specimen dilution (Sample Buffer)
12ml
20×Wash Buffer
50ml
Standards: 10ng/bottle
2 bottles
Substrate working fluid (TMB Solution)
12ml
Primary antibody working solution (Biotinylated Antibody)
12ml
Stop Solution
12ml
Prepare reagents and collect blood samples
1. Collection of specimens: serum, plasma (EDTA, citrate, heparin anticoagulation), cell culture supernatant, tissue homogenate, etc., as early as possible, stored at 2-8 ° C for 48 hours; longer time must be frozen (-20 °C or -70 °C) Save to avoid repeated freezing and thawing. Serum and plasma were diluted 1 : 2 (take 100 ul, add 100 ul of standard dilution, dilute 2 times). The cell culture supernatant can be directly detected without dilution.
2. Standard solution preparation: Add 1 ml of distilled water before use and mix well to prepare a 10 ng/ml solution. Set the standard tube 8 tube, the first tube plus the standard dilution 900ul, the second to the eighth tube to add the sample dilution 500ul. Add 10 ng of the standard solution of 10 ng/ml to the first tube, mix and aspirate 500 ul with a pipette and transfer to the second tube. Repeat the dilution as described above, and remove 500 ul from the seventh tube and discard it. The eighth tube is a blank control.
3. The 10× specimen dilution was diluted 1:10 with distilled water (example: 1 ml concentrated dilution + 9 ml distilled water).
4. Washing solution: diluted 1:20 with distilled water (example: 1 ml concentrated washing solution added to 19 ml of distilled water)
Test procedure
1. Loading: Add 100 ul of standard or sample to be tested in each well. Mix the reaction plate thoroughly and let it stand at 37 °C for 120 minutes.
2. Wash the plate: Wash the plate thoroughly with washing solution 4-6 times, and dry it on the filter paper.
3. Add 100 ul of the first antibody working solution to each well. The reaction plate was thoroughly mixed and placed at 37 ° C for 60 minutes.
4. Wash the board: the same as before.
5. Add 100 ul of enzyme-labeled antibody working solution per well. The reaction plate was placed at 37 ° C for 30 minutes.
6. Wash the board: same as before.
7. Add 100 ul of substrate working solution per well, set 37 The reaction was carried out in the dark at °C for 15 minutes.
8. Add 100 ul of stop solution to each well and mix.
9. Measure the absorbance at 450 nm using a microplate reader within 30 minutes.
Result calculation and judgment
1. All OD values ​​should be subtracted from the blank value before calculation.
2. Using standard products 1000, 500, 250, 125, 62.5, 31.2, 15.6, 0 pg/ml as the abscissa and OD as the ordinate, plot on the coordinate paper and draw the standard curve.
3. Find the corresponding b-Amyloid 1-42 content on the graph based on the OD value of the sample, and multiply by the dilution factor.
Kit performance
1. Sensitivity: The minimum b-Amyloid 1-42 assay concentration is less than 8pg/ml.
2. Specificity: Recombinant or natural human b-Amyloid 1-42 can be detected simultaneously. Does not cross-react with other human cytokines.
3. Repeatability: The coefficient of variation in the plate and plate is less than 12%.
Precautions
1. It is recommended to make double holes for the above standard holes and samples to be tested. The standard curve should be made at the same time for each measurement.
2. The washing process is critical. Insufficient washing will result in an accuracy error and an erroneous rise in the OD value.
3. After the slats are opened, the remaining slats should be sealed again to keep the slats dry .
4. This kit should be stored in a 4 o C refrigerator.
5. This kit is for scientific research only and cannot be used for clinical diagnosis!