Inositol, also known as cyclohexanol, is a vitamin drug and a hypolipidemic drug. It can promote fat metabolism in liver and other tissues, and is used for adjuvant treatment of fatty liver and hyperlipidemia. Due to the lack of suitable chromophores and electroactive groups in the inositol molecule, it is difficult to determine directly by conventional analytical methods. The method for determining the inositol content is a gravimetric method, a microbial method, and a potassium periodate oxidation method, and also an HPLC method and a GC method. Microbiological method and gravimetric method are cumbersome and time-consuming. When the content of inositol and its preparation is determined by gravimetric method, there is still a large amount of sample, and it is necessary to remove chloroform after chloroform extraction, which is harmful to health; potassium periodate oxidation method is required Strict control of the heating oxidation time is consistent, otherwise the measurement results will have a large error; and the HPLC method, using the end absorption for detection, the error is large. The inositol is silylated, and the content of inositol is measured by temperature-programmed gas chromatography after extraction with n-hexane. The method is simple and the result is accurate. Gas Chromatographic Determination of Required Instruments and Reagents The gas chromatograph was equipped with a FID detector, a numerically controlled ultrasonic cleaner, and a 25 ml stoppered test tube. Hexane, anhydrous sodium sulfate and dimethylformamide are of analytical grade (dimethyl carbamide needs to be distilled to remove water before use); trimethylchlorosilane, hexamethyldisilazane, inositol control Product, inositol sample. Several aspects to be aware of when measuring: 1) Due to the lack of suitable chromophores and electroactive groups in the inositol molecule, it is difficult to determine directly by the usual analytical methods. Considering the structural characteristics of the inositol cyclic saturated polyhydroxy group, it was selected to be silylated, extracted with n-hexane and subjected to gas chromatography analysis. Several silylating reagents were selected and tested in different proportions. It was determined that dimethylchlorosilane and hexamethyldisilazane were mixed at 1:3 as derivatization reagents, and the effect was better. 2) The gasification temperature is higher after silanization of inositol, and the temperature of the injection chamber is 260 °C. It is found that there are more peaks after silanization of inositol, and the column temperature is too constant when the column temperature is too high. High, the solvent peak does not return to the baseline, the main peak has been out of the column, and the peak is enclosed in it; if the column temperature is too low, the analysis process is long and the peak shape is severely tailed; The peak and the main peak are well separated, and the peak shape is good and the retention time is appropriate. 3) The packed column adopts programmed temperature and the baseline drift is more serious. It is found that two columns of the same type are selected, and the two-channel simultaneous carrier gas can balance and reduce the baseline drift for better measurement. 4) In order to complete the silylation reaction, the dimethylformamide used should be used after vacuum distillation to remove water. The silanization reaction is slow and should be heated appropriately in a water bath to accelerate the silanization reaction rate.