Diethyl ketone production of herring production technology

In the artificial reproduction hatch of “Four Home Fish”, the herring production is difficult, and the inducing rate is generally low, and the fertilization rate and hatching rate are not high. If the pituitary gland is not used in the oxytocin production, it is generally difficult for the herring production to succeed. In actual production, the pituitary gland is relatively expensive, and even if it is the pituitary it picks, its quality is difficult to guarantee. How to find substitutes for the pituitary gland to ensure the success rate of herring production is a problem faced by many production units. According to the actual production practice for many years, the author did not use the pituitary gland in 2004. The artificial production of herring hormones and analogues by artificial synthesis was successful. First, the parent breeding 1. Pond conditions used test fish from the Yangtze River, Jiangsu Yangtze River family fish breeding field of artificial breeding team. The area of ​​aquaculture ponds is 3.5 mu. The depth of the pond is 2 meters. It is convenient for water drainage and the water quality is fresh. 2. Daily management (1) Bait management The herring rearing is mainly based on animal feeds such as snails and clams. When the water temperature reaches 10°C or above, product bait is fed once every 2 to 3 days and fed at 1% to 2% of the body weight of the fish. When the water temperature is above 18°C, the daily feeding amount is increased to the body weight of the fish. 2% to 4%; when the water temperature reaches 22°C or above, the daily feeding amount increases to 5% to 8% of the body weight of the fish. At this time, a large number of animal live foods such as snails and cockroaches should be fed. Feeding should be based on the fish’s eating and drinking conditions, water quality, and weather conditions. (2) Water quality management In the spring and autumn season, water injection is usually 2 to 3 times per month, water injection is 5 to 8 times per month, and water injection is 8 to 12 centimeters per month. In the spring and autumn season, the water is changed once every season, and the water is changed once a month in summer. Each time, the amount of water changed is 1/5 to 1/4 of the original pool water. Second, invigorating production 1. Quantity of production In the morning of May 19, 2004, the catch of the common carp, which has a better maturity, was put into the spawning pool. Take special care when fishing and transporting herring parents to avoid damaging the fish. A total of 10 bluefish were elicited, weighing 207.5 kilograms, including 5 females and 109.5 kilograms; 5 males and 98 kilograms. 2. Effort time for oxytocin production Two-needle injection method is used, and the needle distance is 12 to 14 hours. The time for the first shot is 12:00-13:00 at noon on May 19. The second shot is at 1:00-2:30 in the morning on May 20. 3. Oxidation drugs and doses The oxytocins are hormone analogues (LRH) and domoxone (DOM). The first female was injected with 1 μg of LRH and 0.5 mg of DOM per kilogram, and the male injected dose was 1/4 of the female. The chest cavity injection method was used, and the water temperature at the time of injection was 23°C. After injection into the spawning tank, keep the micro-flowing water. The second female injected LRH 6 micrograms + DOM 5 milligrams per kilogram, and the male injected dose was 1/2 of the female. The method of back muscle injection was used, and the water temperature at the time of injection was 22°C. After being injected into the spawning pool, microfluidic water was maintained, and the water flow was increased after 8 hours. III. Spawning 1. Spawning time At 10:30 am on May 20th, herring began to heat, and the effect time was 10 to 12 hours. At 11:30, the estrus reached its climax. At this time, we began to pull nets for artificial egg collection and insemination. The specific operation steps are as follows: the eggs of the female to be produced are first collected in the basin, and then the male sperm is squeezed in. After the sperm is squeezed into the basin, the basin is shaken immediately to fully combine the sperm and the egg to increase the fertilization rate. Artificial egg collection and insemination continued until 12:30. 2. Incremental productivity The 5 groups of herring communis were induced by eliciting 4 groups of communist pigs. One group had no production, the yield was 80%, and a total of 2.925 million eggs were produced. IV. Incubation and Emergence 1. Fertilized Rate After the fertilized eggs have passed the number, they are put into the No. 8 hatchery ring for artificial hatching. The No. 8 hatching ring is a circular single ring of hatching rings with a volume of 6 cubic meters. After fertilized eggs were placed in the incubation loop for 10 to 12 hours, the fertilization rate was calculated to be 90.5%, and 2.437 million pigs were expected to emerge. 2. Hatching rate At 10:00 am on May 25th, the number of all fry hatchery that can be cultivated in ponds was 2.52 million. The hatching rate was 95.2%. 3. The seedling rate was 2.925 million eggs and 2.52 million seedlings emerged, and the total seedling rate was 88.6%.

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