1. Increase the temperature of the pig house First, the open pig house is covered with plastic film. It is best to cover the double-layer plastic film. Although the cost is higher than that of the single-layer plastic film, the insulation effect is better. This approach is suitable for fat and non-lactating sows. The second is the boiler-type heating supply in closed pig-raising workshops. The third is the open-type heating heating and plastic film covering the piggery. The fourth is heating heating plus electric heater, suitable for nursing sows. 2. The outer wall of the back wall, gable, and front wall of a thick and strict pig house should be increased in winter. The crop stalks can be stacked and the adobe can be temporarily built. It is best to thicken the outer walls when the pig house is built. 3. The ground to dry the fecal sewage should be promptly removed and cleaned. In order to facilitate drying, the piggery should be made of concrete floor. 4. Addition of bed plate in the pig stage, where conditions permit, it is best to use wood, bamboo, thick fiberboard and other warm bed, without conditions can be laid cement prefabricated panels. 5. Improve the temperature of drinking water Conditionally, you can make cold water boiled in warm water and let the pigs drink, or extract deep groundwater to feed the pigs. 6. Increase the number of feedings The purpose of increasing the number of feedings is to relatively increase the feed intake to compensate for the weight loss caused by the body fat and intramuscular metabolic heat production caused by the low temperature. Where conditions permit, it is best to take free food. Feeding can be added at night in places where feeding is scheduled. 7. Increase the energy level of feed can be increased by about 10% based on the original feed energy level. The big pigs are low and the pigs are high. Piglets can be supplemented with high-energy feeds for fats and oils. Big pigs can increase the amount of common energy feeds such as corn in batches. 8. Increase lighting area in pig houses Open pig houses must have a large open area in order to prepare plastic coverings for winter lighting. Closed-type pig houses are to be reserved in the south wall and roofs for winter installation of glass
The enzyme-linked immunosorbent assay (ELISA) (/ɪˈlaɪzÉ™/, /ËŒiËˈlaɪzÉ™/) is a commonly used analytical biochemistry assay, first described by Engvall and Perlmann in 1971.[1] The assay uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measured. ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries.
In the most simple form of an ELISA, antigens from the sample are attached to a surface. Then, a matching antibody is applied over the surface so it can bind to the antigen. This antibody is linked to an enzyme, and in the final step, a substance containing the enzyme's substrate is added. The subsequent reaction produces a detectable signal, most commonly a color change.
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