Using modern biotechnology to cultivate snow lotus

Saussurea is a valuable plant medicinal material, mainly grown in Gansu, Qinghai, Tibet and other areas with an altitude of 4,000 to 5,000 meters. Therefore, it takes a long period of growth and usually takes 5 years to grow into mature plants (about 40 to 50 Centimeters long). The breeding of snow lotus must wait until the ripening results, and then rely on the wind to blow the seeds to suitable areas for growth, complete the reproduction of the species. In folk snow lotus used to treat rheumatoid arthritis, impotence, irregular menstruation in women, fetal clothing and other symptoms. In medicine, it is mainly used for the treatment of cerebral arteriosclerosis, cancer, etc. In addition, Xuelian can also be used to extract injections and develop rheumatic capsules (these drugs are popular in Hong Kong and Southeast Asia and other regions and countries). Saussurea also has the functions of spreading blood and impotence, impotence, and replenishing blood, delaying aging, and has broad prospects for development in family planning and cosmetics. The cosmetics series (skin cream, sunscreen cream, and children's cream) developed from Saussurea Volatile Oil contains conditional nutrition and active ingredients, has good permeability, is easy to absorb water, and has a long effect, and can significantly delay skin aging, nourish skin cells, and increase skin elasticity. The product is China's first cosmetics that won the invention prize of the Paris International Invention Fair. Saussurea multi-drug function, the use of different polar components of the active ingredients can be considered to add the use of Saussurea cultivation, such as polysaccharide termination of pregnancy and anti-fatigue, anti-hypoxia, anti-inflammatory, analgesic, depression and other effects. Wild Saussurea is in short supply Saussurea is a wild plant that grows in cold regions and has not been discovered by people for a long time, let alone its use value. This is often the case in societies. When people's living standards have increased to a certain degree, people have the ability to discover and explore certain areas of unknowability. It may have been discovered in this situation. Before the middle of the 20th century, only ethnic minorities learned about the medicinal value of Saussurea. As people's understanding of Saussurea medicinal value increased, the market demand became larger and larger, and people started consciously large-scale cultivation. Snow lotus to meet the needs of life. We know that Saussurea has a long growth cycle, usually about 5 years, coupled with chaos and indiscriminate digging, which causes Saussurea to “supply shortages,” leading to the destruction of species resources.
The reporter learned from relevant departments that the data from the market survey in 1986 showed that at that time, the amount of snow lotus in Xinjiang should be taken as 5 tons per year. At present, the demand for snow lotus in Xinjiang's major pharmaceutical plants is more than 100 tons per year. This is how "in short supply"! At present, due to a large number of excavations, Saussurea has reached the point where it is in an impending situation. On the one hand, there is a severe shortage of resources. On the other hand, prices have risen sharply. On the Xinjiang market alone, Saussurea sells for 10 to 15 yuan each. The State Council once stated that it is necessary to limit the excavation of wild plants such as Saussurea. Due to the lack of manpower and material resources during the implementation of law enforcement personnel, there is still the phenomenon of chaos. The Xinjiang Grassland Supervision Station recommends that, in the transitional area of ​​excavation and digging, the closure should be blocked as soon as possible, and snow lotus should be banned for 5-10 years. In the forbidden mining period, all collection, acquisition, and business activities of Saussurea are forbidden. Scientific research shows that traditional large-scale cultivation of snow lotus is often affected by high areas, the environment, and the climate. It is not easy to control the quality of drugs. If taking and injection of saussurea drugs for a long time, it will bring great hidden dangers to people's health. At the same time, Saussurea grows slowly, occupying a large area of ​​farmland for a long time, which is not conducive to "turnover" of cultivated land and affects agricultural production. Therefore, relying on traditional Saussurea cultivation to develop the Chinese medicine industry is not worth the candle. Modern biotechnology to achieve the industrialization of Saussurea Modern planting of Saussurea morifolium is the use of modern biotechnology to produce Saussurea in cell culture. With the rapid development of large-scale plant cell culture technology, modern bioreactor technology, and separation and purification technology, biotechnology has opened up new ways to solve major issues such as resources and energy, environment and health that human beings face, and its application range has spread. In various fields such as medicine, health and environmental protection, a number of emerging industries have gradually emerged. Dr. Zhao Dexiu, a researcher at the Institute of Botany, Chinese Academy of Sciences, is currently conducting research on the production of effective components of flavonoids in large quantities of Saussurea cells. He has published more than 10 papers related to the research of this project and has applied for 2 national patents. According to Dr. Zhao Dexiu, at present, there are three main methods for cell culture of Saussurea. One is the solid culture of Saussurea purpurea. This culture is mainly used to visually screen high-yielding flavonoid cell lines that grow under solid conditions; one is Saussurea Suspension Culture of Cells: High-yield flavonoid cell lines obtained by laboratory screening are suspended in a shaker; another is the fermentation culture of Saussurea cells: This culture is mainly carried out by a bioreactor. One of the keys to industrialization is the use of modern biotechnology to produce Saussurea for cell culture of Saussurea, which has high-yield, stable-producing cells. The high-producing flavonoid cell line A?1 is poorly aggregated in cell suspension culture and is suitable for solid culture. The obtained flavonoid prolific cell line A?2 is characterized by stable genetic properties and high flavonoid content, and cell suspension culture tends to form cell aggregates. The body is more suitable for large-scale production of large-scale bioreactors. Dr. Zhao and his students have successfully completed the research on the technological conditions of the 5-liter bioreactor. The next step is to prepare a 20-liter bioreactor for the saury culture pilot trial in collaboration with Beijing Jinxiu Earth Agriculture Co., Ltd. Dr. Zhao told reporters. “In two years, cell culture will produce Saussurea medicinal components that will be industrialized. The Saussurea cell culture bioreactor scale can reach 200 liters. Our goal is to obtain the batch number of saussurea biopharmaceuticals and put them into production.” Saussurea's huge economy The prospects of doing anything have to consider its economic benefits, and scientific research is no exception. Saussurea has a large economic benefit. Studies have shown that Saussurea ssp. flavonoid cell line has a stable genetic property, a high content of active ingredients in the culture, and a short fermentation period. It is proved by modern chemical analysis methods and preliminary pharmacological and acute toxicity experiments that saussurea bacteria culture can produce The effective secondary production of wild plants, as well as the discovery of no toxic substances, this study laid the foundation for the large-scale cultivation of snow lotus and the industrial production of effective secondary products. The Saussurea Injection and Saussurea Rheumatoid Capsule developed by Saussurea wilsonii have a good effect on the treatment of rheumatism and hemiplegia.
Alpine Saussurea has a special growth environment, and its cultivation is difficult to grow. It takes 3-5 years to mature, and the price is expensive. Snow lotus is on the verge of extinction. The active components of Saussurea have complex chemical structures and are difficult to synthesize chemically. The large-scale production of botanicals by plant cells is not limited by natural conditions, and drug quality is easily controlled without occupying the area of ​​cultivated land. The use of cell culture to produce Saussurea flavonoids raw materials not only protects wild resources, maintains ecological balance, but also greatly reduces costs. The biggest feature of large-scale cultivation of plant cells is that they do not occupy arable land, but China is a populous country and the per capita arable land is relatively small. Therefore, with the improvement of people’s living standards, modern biotechnology is used to develop China’s pharmaceutical production and change the product structure. ,Significant. Biotechnology makes Alpine Saussurea no longer reachable.

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